Low-ionic-strength saline solution-antiglobulin test (LISS-AGT)
نویسندگان
چکیده
منابع مشابه
Use of low ionic strength saline for crossmatching and antibody screening.
A totally revised crossmatching and antibody screening procedure has been designed using low ionic strength saline as the suspending medium for red cells in the saline and enzyme phases as well as the antiglobulin test. The replacement of Pasteur pipettes with precision samplers for all serum and red cell dispensing has resulted in improvement in the reproducibility and standardisation of techn...
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BACKGROUND AND OBJECTIVES We recently demonstrated that direct antiglobulin titer (DAT) positive, blood group A or B newborns born to group O mothers had a high incidence of hyperbilirubinemia, attributable to increased hemolysis. We reanalyzed our data asking whether increasing DAT strength plays a modulating role in the pathophysiology of the hemolysis and hyperbilirubinemia. METHODS Data f...
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The effect of ionic activity on the quenching of the quinine dication fluorescence by chloride and bromide ions is examined in solutions of sufficiently low ionic strength to enable ionic activities to be taken into account by means of the Debye-Hückel equation. An equation for the dependence of the Stern-Volmer constant on the ionic strength is derived in a novel way and an iterative, linear l...
متن کاملLow Ionic Strength Buffer for One Litre
SYNOPSIS Erythrocytes may be coated with blood group antibodies with or without reacting complement or sometimes apparently with complement alone. This may occur in vivo in such conditions as autoimmune acquired haemolytic anaemia, haemolytic disease of the newborn, or after transfusions of incompatible blood. It may occur in vitro also by the deliberate sensitization of erythrocytes during lab...
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The interaction of actin with myosin and heavy meromyosin was studied in the presence of Mgf+ ion and adenosine triphosphate at low ionic strength. When the heavy meromyosin ATPase was 17-fold activated by actin, the actoheavy meromyosin complex responsible for this activation did not give rise to a measurable increase in the viscosity of the sample above that predicted for a noninteracting mix...
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ژورنال
عنوان ژورنال: Immunohematology
سال: 2019
ISSN: 0894-203X,1930-3955
DOI: 10.21307/immunohematology-2018-010